Department of Biochemistry University of Oxford Department of Biochemistry
University of Oxford
South Parks Road
Oxford OX1 3QU

Tel: +44 (0)1865 613200
Fax: +44 (0)1865 613201
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Anthony Watts
Resolving structural details of membrane peptides and proteins at high resolution

Co-workers: Roslin Adamson, Juan Bada, Juan Bolivar Gonzalez , Claudia Cassidy, Hugh Dannatt, Charles Davies, Patricia Dijkman, James Harris, Rosana Inácio dos Reis, Peter Fisher, Peter Judge, Steven Lavington, Matylda Maciejewska, Juan Munoz-Garcia, Marc-Philipp Pfeil, Garrick Taylor, Samantha Woerner and Daniel Yin.

 

Resolving structural details of membrane receptors is still a major challenge, not least because of the difficulties of expression, crystallization and their size. Since membrane protein secondary structure can be modelled for many cases, we have been devising solid state NMR methods for determining high resolution (sub-Å) details of information-rich sites within membrane receptors (Watts, A., 2005. Nature Reviews Drug Discovery, 4, 555-568). In particular, we have resolved new information about ligand (drug, neurotransmitter or solute) binding sites, and related all this information to functional descriptions.

Our recent focus has been on the neurotensin receptor (NTS1) which we have expressed in E. coli in structural biology amounts (Attrill et al, 2009, J. Express & Purif, 64, 32-38) in a functionally competent form for structural studies, some of which involve single molecule approaches for bionanotechnological and drug design applications.

NTS1 is now available highly purified monodispersed in detergent and in a ligand-binding form. One approach to monitoring ligand binding has been to develop a novel surface plasmon resonance method for tagging the natural ligand, neurotensin (13-mer peptide), to the chip and monitoring protein binding (Harding et al., 2006, Euro. Biophys. J., 35, 709-712). Fluorescently tagged NTS1 has also been used in fluorescence resonance energy transfer methods to resolve long range information of protein-protein signalling (Harding, et al., 2009, Biophsy. J., 96, 964-973).

Publications - 2014

  • Goddard, A., Dijkman, P.M., Adamson, R.J., dos Reis, R.I. and Watts, A. (2014) Reconstitution of membrane proteins with a GPCR as an exampleMethods in Enzymology (in press)
  • Adamson, R. and Watts, A. (2014) Kinetics of the early events of GPCR signalling, FEBS Letts, (in press)
  • Liebel M, Schnedermann C, Bassolino G, Taylor G, Watts A, Kukura P (2014) Direct observation of the coherent nuclear response after the absorption of a photon. Physical Review Letters. ; 112, 238301
  • Judge, P.J., Taylor, G. F.,Vermeer, L. S. and  Watts A. (2014), "Structural Insights from Solid-State NMR into the Function of the Bacteriorhodopsin Photoreceptor Protein" CHAPTER 23 in Advances in Biological Solid-State NMR: Proteins and Membrane-Active Peptides, (eds  F.  Separovic, A. Naito)

Publications - 2013

Publications - 2012

More Publications...

A Fascination with Vision - a video lecture for non-specialists

Research Images






Figure 1: Substituted imidazo-pyridines are inhibitors of the gastric H+/K+-ATPase. By specific labelling of members of this drug family with NMR visible isotopes, we have been able to define the full conformation of the bound ligand, and suggest mechanism for inhibition from homology modelling with a related protein. (Kim, Watts & Watts, 2005, J. Med. Chem. 48, 7145-7152 and Watts, Watts & Middleton, 2001, J. Biol. Chem., 276, 43197-43204).


Figure 2: The cation-π interaction of acetyl choline, a major brain neurotransmitter, and the ligand gated, nicotinic acetyl choline receptor has been resolved using solid state NMR, giving an insight into the binding mechanism and the residues surrounding the site. (Watts, 2005, Nature Reviews Drug Discovery, 4, 555-568; Williamson et al., 2007, PNAS, 104, 18031-18036)

Figure 3: The way in which retinal is restrained within its binding site in membrane-embedded mammalian rhodopsin, and the structural details of the site, have been resolved for the early activation states of this light-activated GPCR, using high resolution solid state NMR to measure atomic distances within the retinal to high accuracy (+/- 0.2Å). (Spooner, et al., 2004, J. Mol. Biol., 343, 719-730)


Contact:anthony.watts@bioch.ox.ac.uk
Graduate Student and Postdoctoral Positions: Enquiries with CV welcome
Website: www.bioch.ox.ac.uk/~oubsu