Publication List

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1: Rajasekar KV, Zdanowski K, Yan J, Hopper JT, Francis ML, Seepersad C, Sharp C,
Pecqueur L, Werner JM, Robinson CV, Mohammed S, Potts JR, Kleanthous C. The
anti-sigma factor RsrA responds to oxidative stress by reburying its hydrophobic
core. Nat Commun. 2016 Jul 19;7:12194. doi: 10.1038/ncomms12194. PubMed PMID:
27432510; PubMed Central PMCID: PMC4960319.


2: Klein A, Wojdyla JA, Joshi A, Josts I, McCaughey LC, Housden NG, Kaminska R,
Byron O, Walker D, Kleanthous C. Structural and biophysical analysis of nuclease
protein antibiotics. Biochem J. 2016 Sep 15;473(18):2799-812. doi:
10.1042/BCJ20160544. Epub 2016 Jul 8. PubMed PMID: 27402794.


3: McCaughey LC, Josts I, Grinter R, White P, Byron O, Tucker NP, Matthews JM,
Kleanthous C, Whitchurch CB, Walker D. Discovery, characterization and in vivo
activity of pyocin SD2, a protein antibiotic from Pseudomonas aeruginosa. Biochem
J. 2016 Aug 1;473(15):2345-58. doi: 10.1042/BCJ20160470. Epub 2016 Jun 1. PubMed
PMID: 27252387; PubMed Central PMCID: PMC4964976.


4: Gault J, Donlan JA, Liko I, Hopper JT, Gupta K, Housden NG, Struwe WB, Marty
MT, Mize T, Bechara C, Zhu Y, Wu B, Kleanthous C, Belov M, Damoc E, Makarov A,
Robinson CV. High-resolution mass spectrometry of small molecules bound to
membrane proteins. Nat Methods. 2016 Apr;13(4):333-6. doi: 10.1038/nmeth.3771.
Epub 2016 Feb 22. PubMed PMID: 26901650; PubMed Central PMCID: PMC4856209.


5: Kleanthous C, Rassam P, Baumann CG. Protein-protein interactions and the
spatiotemporal dynamics of bacterial outer membrane proteins. Curr Opin Struct
Biol. 2015 Dec;35:109-15. doi: 10.1016/j.sbi.2015.10.007. Epub 2015 Nov 26.
Review. PubMed PMID: 26629934; PubMed Central PMCID: PMC4684144.


6: Kleanthous C, Armitage JP. The bacterial cell envelope. Philos Trans R Soc
Lond B Biol Sci. 2015 Oct 5;370(1679). pii: 20150019. doi:
10.1098/rstb.2015.0019. PubMed PMID: 26370932; PubMed Central PMCID: PMC4632596.


7: Wojdyla JA, Cutts E, Kaminska R, Papadakos G, Hopper JT, Stansfeld PJ,
Staunton D, Robinson CV, Kleanthous C. Structure and function of the Escherichia
coli Tol-Pal stator protein TolR. J Biol Chem. 2015 Oct 30;290(44):26675-87. doi:
10.1074/jbc.M115.671586. Epub 2015 Sep 9. PubMed PMID: 26354441; PubMed Central
PMCID: PMC4646322.


8: Joshi A, Grinter R, Josts I, Chen S, Wojdyla JA, Lowe ED, Kaminska R, Sharp C,
McCaughey L, Roszak AW, Cogdell RJ, Byron O, Walker D, Kleanthous C. Structures
of the Ultra-High-Affinity Protein-Protein Complexes of Pyocins S2 and AP41 and
Their Cognate Immunity Proteins from Pseudomonas aeruginosa. J Mol Biol. 2015 Aug
28;427(17):2852-66. doi: 10.1016/j.jmb.2015.07.014. Epub 2015 Jul 26. PubMed
PMID: 26215615; PubMed Central PMCID: PMC4548480.


9: Rassam P, Copeland NA, Birkholz O, Tóth C, Chavent M, Duncan AL, Cross SJ,
Housden NG, Kaminska R, Seger U, Quinn DM, Garrod TJ, Sansom MS, Piehler J,
Baumann CG, Kleanthous C. Supramolecular assemblies underpin turnover of outer
membrane proteins in bacteria. Nature. 2015 Jul 16;523(7560):333-6. doi:
10.1038/nature14461. Epub 2015 Jun 10. PubMed PMID: 26061769; PubMed Central
PMCID: PMC4905513.


10: Papadakos G, Sharma A, Lancaster LE, Bowen R, Kaminska R, Leech AP, Walker D,
Redfield C, Kleanthous C. Consequences of inducing intrinsic disorder in a
high-affinity protein-protein interaction. J Am Chem Soc. 2015 Apr
29;137(16):5252-5. doi: 10.1021/ja512607r. Epub 2015 Apr 17. PubMed PMID:
25856265.


11: Lensink MF, Moal IH, Bates PA, Kastritis PL, Melquiond AS, Karaca E, Schmitz
C, van Dijk M, Bonvin AM, Eisenstein M, Jiménez-García B, Grosdidier S, Solernou
A, Pérez-Cano L, Pallara C, Fernández-Recio J, Xu J, Muthu P, Praneeth Kilambi K,
Gray JJ, Grudinin S, Derevyanko G, Mitchell JC, Wieting J, Kanamori E, Tsuchiya
Y, Murakami Y, Sarmiento J, Standley DM, Shirota M, Kinoshita K, Nakamura H,
Chavent M, Ritchie DW, Park H, Ko J, Lee H, Seok C, Shen Y, Kozakov D, Vajda S,
Kundrotas PJ, Vakser IA, Pierce BG, Hwang H, Vreven T, Weng Z, Buch I, Farkash E,
Wolfson HJ, Zacharias M, Qin S, Zhou HX, Huang SY, Zou X, Wojdyla JA, Kleanthous
C, Wodak SJ. Blind prediction of interfacial water positions in CAPRI. Proteins.
2014 Apr;82(4):620-32. doi: 10.1002/prot.24439. Epub 2013 Nov 23. PubMed PMID:
24155158; PubMed Central PMCID: PMC4582081.


12: Vankemmelbeke M, Housden NG, James R, Kleanthous C, Penfold CN. Immunity
protein release from a cell-bound nuclease colicin complex requires global
conformational rearrangement. Microbiologyopen. 2013 Oct;2(5):853-61. doi:
10.1002/mbo3.122. Epub 2013 Aug 28. PubMed PMID: 24039240; PubMed Central PMCID:
PMC3831645.


13: Housden NG, Hopper JT, Lukoyanova N, Rodriguez-Larrea D, Wojdyla JA, Klein A,
Kaminska R, Bayley H, Saibil HR, Robinson CV, Kleanthous C. Intrinsically
disordered protein threads through the bacterial outer-membrane porin OmpF.
Science. 2013 Jun 28;340(6140):1570-4. doi: 10.1126/science.1237864. PubMed PMID:
23812713; PubMed Central PMCID: PMC3856478.


14: Farrance OE, Hann E, Kaminska R, Housden NG, Derrington SR, Kleanthous C,
Radford SE, Brockwell DJ. A force-activated trip switch triggers rapid
dissociation of a colicin from its immunity protein. PLoS Biol.
2013;11(2):e1001489. doi: 10.1371/journal.pbio.1001489. Epub 2013 Feb 19. PubMed
PMID: 23431269; PubMed Central PMCID: PMC3576412.


15: Housden NG, Kleanthous C. Colicin translocation across the Escherichia coli
outer membrane. Biochem Soc Trans. 2012 Dec 1;40(6):1475-9. doi:
10.1042/BST20120255. Review. PubMed PMID: 23176501.


16: Penfold CN, Walker D, Kleanthous C. How bugs kill bugs: progress and
challenges in bacteriocin research. Biochem Soc Trans. 2012 Dec 1;40(6):1433-7.
doi: 10.1042/BST20120253. PubMed PMID: 23176494.


17: Papadakos G, Housden NG, Lilly KJ, Kaminska R, Kleanthous C. Kinetic basis
for the competitive recruitment of TolB by the intrinsically disordered
translocation domain of colicin E9. J Mol Biol. 2012 May 18;418(5):269-80. doi:
10.1016/j.jmb.2012.01.039. Epub 2012 Jan 30. PubMed PMID: 22310049.


18: Wojdyla JA, Fleishman SJ, Baker D, Kleanthous C. Structure of the
ultra-high-affinity colicin E2 DNase--Im2 complex. J Mol Biol. 2012 Mar
16;417(1-2):79-94. doi: 10.1016/j.jmb.2012.01.019. Epub 2012 Jan 27. PubMed PMID:
22306467.


19: Pashley CL, Morgan GJ, Kalverda AP, Thompson GS, Kleanthous C, Radford SE.
Conformational properties of the unfolded state of Im7 in nondenaturing
conditions. J Mol Biol. 2012 Feb 17;416(2):300-18. doi:
10.1016/j.jmb.2011.12.041. Epub 2011 Dec 28. PubMed PMID: 22226836; PubMed
Central PMCID: PMC3314952.


20: Papadakos G, Wojdyla JA, Kleanthous C. Nuclease colicins and their immunity
proteins. Q Rev Biophys. 2012 Feb;45(1):57-103. doi: 10.1017/S0033583511000114.
Epub 2011 Nov 16. Review. PubMed PMID: 22085441.


21: Housden NG, Kleanthous C. Thermodynamic dissection of colicin interactions.
Methods Enzymol. 2011;488:123-45. doi: 10.1016/B978-0-12-381268-1.00006-9.
Review. PubMed PMID: 21195227.


22: Housden NG, Wojdyla JA, Korczynska J, Grishkovskaya I, Kirkpatrick N,
Brzozowski AM, Kleanthous C. Directed epitope delivery across the Escherichia
coli outer membrane through the porin OmpF. Proc Natl Acad Sci U S A. 2010 Dec
14;107(50):21412-7. doi: 10.1073/pnas.1010780107. Epub 2010 Nov 22. PubMed PMID:
21098297; PubMed Central PMCID: PMC3003033.


23: Kleanthous C. Swimming against the tide: progress and challenges in our
understanding of colicin translocation. Nat Rev Microbiol. 2010 Dec;8(12):843-8.
doi: 10.1038/nrmicro2454. Epub 2010 Nov 9. Review. PubMed PMID: 21060316.


24: Ng CL, Lang K, Meenan NA, Sharma A, Kelley AC, Kleanthous C, Ramakrishnan V.
Structural basis for 16S ribosomal RNA cleavage by the cytotoxic domain of
colicin E3. Nat Struct Mol Biol. 2010 Oct;17(10):1241-6. doi: 10.1038/nsmb.1896.
Epub 2010 Sep 19. PubMed PMID: 20852642; PubMed Central PMCID: PMC3755339.


25: Krachler AM, Sharma A, Cauldwell A, Papadakos G, Kleanthous C. TolA modulates
the oligomeric status of YbgF in the bacterial periplasm. J Mol Biol. 2010 Oct
22;403(2):270-85. doi: 10.1016/j.jmb.2010.08.050. Epub 2010 Sep 15. PubMed PMID:
20816983.


26: Meenan NA, Sharma A, Fleishman SJ, Macdonald CJ, Morel B, Boetzel R, Moore
GR, Baker D, Kleanthous C. The structural and energetic basis for high
selectivity in a high-affinity protein-protein interaction. Proc Natl Acad Sci U
S A. 2010 Jun 1;107(22):10080-5. doi: 10.1073/pnas.0910756107. Epub 2010 May 17.
PubMed PMID: 20479265; PubMed Central PMCID: PMC2890441.


27: Krachler AM, Sharma A, Kleanthous C. Self-association of TPR domains: Lessons
learned from a designed, consensus-based TPR oligomer. Proteins. 2010
Jul;78(9):2131-43. doi: 10.1002/prot.22726. PubMed PMID: 20455268.


28: Kleanthous C. Translocator hunt comes full Cir-Col. Mol Microbiol. 2010
Feb;75(3):529-33. doi: 10.1111/j.1365-2958.2009.06967.x. Epub 2009 Dec 23. PubMed
PMID: 20041993.


29: Levin KB, Dym O, Albeck S, Magdassi S, Keeble AH, Kleanthous C, Tawfik DS.
Following evolutionary paths to protein-protein interactions with high affinity
and selectivity. Nat Struct Mol Biol. 2009 Oct;16(10):1049-55. doi:
10.1038/nsmb.1670. Epub 2009 Sep 13. PubMed PMID: 19749752.


30: Bonsor DA, Hecht O, Vankemmelbeke M, Sharma A, Krachler AM, Housden NG, Lilly
KJ, James R, Moore GR, Kleanthous C. Allosteric beta-propeller signalling in TolB
and its manipulation by translocating colicins. EMBO J. 2009 Sep
16;28(18):2846-57. doi: 10.1038/emboj.2009.224. Epub 2009 Aug 20. Erratum in:
EMBO J. 2009 Sep 16;28(18):2858. PubMed PMID: 19696740; PubMed Central PMCID:
PMC2750012.


31: Vankemmelbeke M, Zhang Y, Moore GR, Kleanthous C, Penfold CN, James R.
Energy-dependent immunity protein release during tol-dependent nuclease colicin
translocation. J Biol Chem. 2009 Jul 10;284(28):18932-41. doi:
10.1074/jbc.M806149200. Epub 2009 May 19. PubMed PMID: 19458090; PubMed Central
PMCID: PMC2707214.


32: Bonsor DA, Meenan NA, Kleanthous C. Colicins exploit native disorder to gain
cell entry: a hitchhiker's guide to translocation. Biochem Soc Trans. 2008
Dec;36(Pt 6):1409-13. doi: 10.1042/BST0361409. Review. PubMed PMID: 19021565.


33: Lancaster LE, Savelsbergh A, Kleanthous C, Wintermeyer W, Rodnina MV. Colicin
E3 cleavage of 16S rRNA impairs decoding and accelerates tRNA translocation on
Escherichia coli ribosomes. Mol Microbiol. 2008 Jul;69(2):390-401. doi:
10.1111/j.1365-2958.2008.06283.x. PubMed PMID: 18485067; PubMed Central PMCID:
PMC2615495.


34: Keeble AH, Joachimiak LA, Maté MJ, Meenan N, Kirkpatrick N, Baker D,
Kleanthous C. Experimental and computational analyses of the energetic basis for
dual recognition of immunity proteins by colicin endonucleases. J Mol Biol. 2008
Jun 13;379(4):745-59. doi: 10.1016/j.jmb.2008.03.055. Epub 2008 Apr 3. PubMed
PMID: 18471830.


35: Cartwright AM, Lim EK, Kleanthous C, Bowles DJ. A kinetic analysis of
regiospecific glucosylation by two glycosyltransferases of Arabidopsis thaliana:
domain swapping to introduce new activities. J Biol Chem. 2008 Jun
6;283(23):15724-31. doi: 10.1074/jbc.M801983200. Epub 2008 Mar 31. PubMed PMID:
18378673; PubMed Central PMCID: PMC3259630.


36: Botër M, Amigues B, Peart J, Breuer C, Kadota Y, Casais C, Moore G,
Kleanthous C, Ochsenbein F, Shirasu K, Guerois R. Structural and functional
analysis of SGT1 reveals that its interaction with HSP90 is required for the
accumulation of Rx, an R protein involved in plant immunity. Plant Cell. 2007
Nov;19(11):3791-804. Epub 2007 Nov 21. PubMed PMID: 18032631; PubMed Central
PMCID: PMC2174866.


37: Nyarko A, Mosbahi K, Rowe AJ, Leech A, Boter M, Shirasu K, Kleanthous C.
TPR-Mediated self-association of plant SGT1. Biochemistry. 2007 Oct
9;46(40):11331-41. Epub 2007 Sep 18. PubMed PMID: 17877371.


38: Walker D, Mosbahi K, Vankemmelbeke M, James R, Kleanthous C. The role of
electrostatics in colicin nuclease domain translocation into bacterial cells. J
Biol Chem. 2007 Oct 26;282(43):31389-97. Epub 2007 Aug 24. PubMed PMID: 17720814.


39: Bonsor DA, Grishkovskaya I, Dodson EJ, Kleanthous C. Molecular mimicry
enables competitive recruitment by a natively disordered protein. J Am Chem Soc.
2007 Apr 18;129(15):4800-7. Epub 2007 Mar 22. PubMed PMID: 17375930.


40: Hann E, Kirkpatrick N, Kleanthous C, Smith DA, Radford SE, Brockwell DJ. The
effect of protein complexation on the mechanical stability of Im9. Biophys J.
2007 May 1;92(9):L79-81. Epub 2007 Mar 9. PubMed PMID: 17351013; PubMed Central
PMCID: PMC1852351.


41: Cascales E, Buchanan SK, Duché D, Kleanthous C, Lloubès R, Postle K, Riley M,
Slatin S, Cavard D. Colicin biology. Microbiol Mol Biol Rev. 2007
Mar;71(1):158-229. Review. PubMed PMID: 17347522; PubMed Central PMCID:
PMC1847374.


42: Heise CT, Le Duff CS, Boter M, Casais C, Airey JE, Leech AP, Amigues B,
Guerois R, Moore GR, Shirasu K, Kleanthous C. Biochemical characterization of
RAR1 cysteine- and histidine-rich domains (CHORDs): a novel class of
zinc-dependent protein-protein interaction modules. Biochemistry. 2007 Feb
13;46(6):1612-23. Epub 2007 Jan 10. PubMed PMID: 17279625.


43: White GF, Ottignon L, Georgiou T, Kleanthous C, Moore GR, Thomson AJ,
Oganesyan VS. Analysis of nitroxide spin label motion in a protein-protein
complex using multiple frequency EPR spectroscopy. J Magn Reson. 2007
Apr;185(2):191-203. Epub 2006 Dec 16. PubMed PMID: 17218133.


44: Loftus SR, Walker D, Maté MJ, Bonsor DA, James R, Moore GR, Kleanthous C.
Competitive recruitment of the periplasmic translocation portal TolB by a
natively disordered domain of colicin E9. Proc Natl Acad Sci U S A. 2006 Aug
15;103(33):12353-8. Epub 2006 Aug 7. PubMed PMID: 16894158; PubMed Central PMCID:
PMC1567883.


45: Zdanowski K, Doughty P, Jakimowicz P, O'Hara L, Buttner MJ, Paget MS,
Kleanthous C. Assignment of the zinc ligands in RsrA, a redox-sensing ZAS protein
from Streptomyces coelicolor. Biochemistry. 2006 Jul 11;45(27):8294-300. PubMed
PMID: 16819828.


46: Keeble AH, Kirkpatrick N, Shimizu S, Kleanthous C. Calorimetric dissection of
colicin DNase--immunity protein complex specificity. Biochemistry. 2006 Mar
14;45(10):3243-54. PubMed PMID: 16519519.


47: Mosbahi K, Walker D, James R, Moore GR, Kleanthous C. Global structural
rearrangement of the cell penetrating ribonuclease colicin E3 on interaction with
phospholipid membranes. Protein Sci. 2006 Mar;15(3):620-7. Epub 2006 Feb 1.
PubMed PMID: 16452623; PubMed Central PMCID: PMC2249781.


48: Housden NG, Loftus SR, Moore GR, James R, Kleanthous C. Cell entry mechanism
of enzymatic bacterial colicins: porin recruitment and the thermodynamics of
receptor binding. Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):13849-54. Epub
2005 Sep 15. PubMed PMID: 16166265; PubMed Central PMCID: PMC1236540.


49: Tozawa K, Macdonald CJ, Penfold CN, James R, Kleanthous C, Clayden NJ, Moore
GR. Clusters in an intrinsically disordered protein create a protein-binding
site: the TolB-binding region of colicin E9. Biochemistry. 2005 Aug
30;44(34):11496-507. PubMed PMID: 16114886.


50: Keeble AH, Kleanthous C. The kinetic basis for dual recognition in colicin
endonuclease-immunity protein complexes. J Mol Biol. 2005 Sep 23;352(3):656-71.
PubMed PMID: 16109424.


51: Vankemmelbeke M, Healy B, Moore GR, Kleanthous C, Penfold CN, James R. Rapid
detection of colicin E9-induced DNA damage using Escherichia coli cells carrying
SOS promoter-lux fusions. J Bacteriol. 2005 Jul;187(14):4900-7. Erratum in: J
Bacteriol. 2005 Sep;187(18):6601. PubMed PMID: 15995205; PubMed Central PMCID:
PMC1169509.


52: van den Heuvel RH, Gato S, Versluis C, Gerbaux P, Kleanthous C, Heck AJ.
Real-time monitoring of enzymatic DNA hydrolysis by electrospray ionization mass
spectrometry. Nucleic Acids Res. 2005 Jun 13;33(10):e96. PubMed PMID: 15956101;
PubMed Central PMCID: PMC1150282.


53: van den Bremer ET, Keeble AH, Kleanthous C, Heck AJ. Metal induced
selectivity in phosphate ion binding in E9 DNase. Chem Commun (Camb). 2005 Mar
7;(9):1137-9. Epub 2005 Jan 13. PubMed PMID: 15726170.


54: Copeland NA, Kleanthous C. The role of an activating peptide in
protease-mediated suicide of Escherichia coli K12. J Biol Chem. 2005 Jan
7;280(1):112-7. Epub 2004 Oct 22. PubMed PMID: 15501819.


55: Macdonald CJ, Tozawa K, Collins ES, Penfold CN, James R, Kleanthous C,
Clayden NJ, Moore GR. Characterisation of a mobile protein-binding epitope in the
translocation domain of colicin E9. J Biomol NMR. 2004 Sep;30(1):81-96. PubMed
PMID: 15452437.


56: Penfold CN, Healy B, Housden NG, Boetzel R, Vankemmelbeke M, Moore GR,
Kleanthous C, James R. Flexibility in the receptor-binding domain of the
enzymatic colicin E9 is required for toxicity against Escherichia coli cells. J
Bacteriol. 2004 Jul;186(14):4520-7. PubMed PMID: 15231784; PubMed Central PMCID:
PMC438598.


57: Copeland NA, Bingham R, Georgiou T, Cooper P, Kleanthous C. Identification of
essential residues within Lit, a cell death peptidase of Escherichia coli K-12.
Biochemistry. 2004 Jun 22;43(24):7948-53. PubMed PMID: 15196039.


58: Maté MJ, Kleanthous C. Structure-based analysis of the metal-dependent
mechanism of H-N-H endonucleases. J Biol Chem. 2004 Aug 13;279(33):34763-9. Epub
2004 Jun 8. PubMed PMID: 15190054.


59: Walker D, Lancaster L, James R, Kleanthous C. Identification of the catalytic
motif of the microbial ribosome inactivating cytotoxin colicin E3. Protein Sci.
2004 Jun;13(6):1603-11. Epub 2004 May 7. PubMed PMID: 15133158; PubMed Central
PMCID: PMC2279995.


60: van den Bremer ET, Keeble AH, Jiskoot W, Spelbrink RE, Maier CS, van Hoek A,
Visser AJ, James R, Moore GR, Kleanthous C, Heck AJ. Distinct conformational
stability and functional activity of four highly homologous endonuclease
colicins. Protein Sci. 2004 May;13(5):1391-401. PubMed PMID: 15096639; PubMed
Central PMCID: PMC2286750.


61: van den Bremer ET, Keeble AH, Visser AJ, van Hoek A, Kleanthous C, Heck AJ,
Jiskoot W. Ligand-induced changes in the conformational dynamics of a bacterial
cytotoxic endonuclease. Biochemistry. 2004 Apr 13;43(14):4347-55. PubMed PMID:
15065879.


62: Mosbahi K, Walker D, Lea E, Moore GR, James R, Kleanthous C. Destabilization
of the colicin E9 Endonuclease domain by interaction with negatively charged
phospholipids: implications for colicin translocation into bacteria. J Biol Chem.
2004 May 21;279(21):22145-51. Epub 2004 Mar 23. PubMed PMID: 15044477.


63: Li W, Keeble AH, Giffard C, James R, Moore GR, Kleanthous C. Highly
discriminating protein-protein interaction specificities in the context of a
conserved binding energy hotspot. J Mol Biol. 2004 Mar 26;337(3):743-59. PubMed
PMID: 15019791.


64: Walker D, Rolfe M, Thompson A, Moore GR, James R, Hinton JC, Kleanthous C.
Transcriptional profiling of colicin-induced cell death of Escherichia coli
MG1655 identifies potential mechanisms by which bacteriocins promote bacterial
diversity. J Bacteriol. 2004 Feb;186(3):866-9. PubMed PMID: 14729715; PubMed
Central PMCID: PMC321497.


65: Li W, Bottrill AR, Bibb MJ, Buttner MJ, Paget MS, Kleanthous C. The Role of
zinc in the disulphide stress-regulated anti-sigma factor RsrA from Streptomyces
coelicolor. J Mol Biol. 2003 Oct 17;333(2):461-72. PubMed PMID: 14529630.


66: Law CJ, Penfold CN, Walker DC, Moore GR, James R, Kleanthous C. OmpF enhances
the ability of BtuB to protect susceptible Escherichia coli cells from colicin E9
cytotoxicity. FEBS Lett. 2003 Jun 19;545(2-3):127-32. PubMed PMID: 12804762.


67: Walker D, Moore GR, James R, Kleanthous C. Thermodynamic consequences of
bipartite immunity protein binding to the ribosomal ribonuclease colicin E3.
Biochemistry. 2003 Apr 15;42(14):4161-71. PubMed PMID: 12680770.


68: Boetzel R, Collins ES, Clayden NJ, Kleanthous C, James R, Moore GR.
Structural dynamics of the receptor-binding domain of colicin E9. Faraday
Discuss. 2003;122:145-62; discussion 171-90. PubMed PMID: 12555855.


69: Kolade OO, Carr SB, Kühlmann UC, Pommer A, Kleanthous C, Bouchcinsky CA,
Hemmings AM. Structural aspects of the inhibition of DNase and rRNase colicins by
their immunity proteins. Biochimie. 2002 May-Jun;84(5-6):439-46. Review. PubMed
PMID: 12423787.


70: James R, Penfold CN, Moore GR, Kleanthous C. Killing of E coli cells by E
group nuclease colicins. Biochimie. 2002 May-Jun;84(5-6):381-9. Review. PubMed
PMID: 12423781.


71: Li W, Stevenson CE, Burton N, Jakimowicz P, Paget MS, Buttner MJ, Lawson DM,
Kleanthous C. Identification and structure of the anti-sigma factor-binding
domain of the disulphide-stress regulated sigma factor sigma(R) from Streptomyces
coelicolor. J Mol Biol. 2002 Oct 18;323(2):225-36. PubMed PMID: 12381317.


72: Keeble AH, Hemmings AM, James R, Moore GR, Kleanthous C. Multistep binding of
transition metals to the H-N-H endonuclease toxin colicin E9. Biochemistry. 2002
Aug 13;41(32):10234-44. PubMed PMID: 12162738.


73: Walker DC, Georgiou T, Pommer AJ, Walker D, Moore GR, Kleanthous C, James R.
Mutagenic scan of the H-N-H motif of colicin E9: implications for the mechanistic
enzymology of colicins, homing enzymes and apoptotic endonucleases. Nucleic Acids
Res. 2002 Jul 15;30(14):3225-34. PubMed PMID: 12136104; PubMed Central PMCID:
PMC135741.


74: van den Bremer ET, Jiskoot W, James R, Moore GR, Kleanthous C, Heck AJ, Maier
CS. Probing metal ion binding and conformational properties of the colicin E9
endonuclease by electrospray ionization time-of-flight mass spectrometry. Protein
Sci. 2002 Jul;11(7):1738-52. PubMed PMID: 12070327; PubMed Central PMCID:
PMC2373645.


75: Collins ES, Whittaker SB, Tozawa K, MacDonald C, Boetzel R, Penfold CN,
Reilly A, Clayden NJ, Osborne MJ, Hemmings AM, Kleanthous C, James R, Moore GR.
Structural dynamics of the membrane translocation domain of colicin E9 and its
interaction with TolB. J Mol Biol. 2002 May 3;318(3):787-804. PubMed PMID:
12054823.


76: Mosbahi K, Lemaître C, Keeble AH, Mobasheri H, Morel B, James R, Moore GR,
Lea EJ, Kleanthous C. The cytotoxic domain of colicin E9 is a channel-forming
endonuclease. Nat Struct Biol. 2002 Jun;9(6):476-84. PubMed PMID: 12021774.


77: Capaldi AP, Kleanthous C, Radford SE. Im7 folding mechanism: misfolding on a
path to the native state. Nat Struct Biol. 2002 Mar;9(3):209-16. PubMed PMID:
11875516.


78: Pommer AJ, Cal S, Keeble AH, Walker D, Evans SJ, Kühlmann UC, Cooper A,
Connolly BA, Hemmings AM, Moore GR, James R, Kleanthous C. Mechanism and cleavage
specificity of the H-N-H endonuclease colicin E9. J Mol Biol. 2001 Dec
7;314(4):735-49. PubMed PMID: 11733993.


79: Kleanthous C, Walker D. Immunity proteins: enzyme inhibitors that avoid the
active site. Trends Biochem Sci. 2001 Oct;26(10):624-31. Review. PubMed PMID:
11590016.


80: Gorski SA, Capaldi AP, Kleanthous C, Radford SE. Acidic conditions stabilise
intermediates populated during the folding of Im7 and Im9. J Mol Biol. 2001 Sep
28;312(4):849-63. PubMed PMID: 11575937.


81: Paget MS, Bae JB, Hahn MY, Li W, Kleanthous C, Roe JH, Buttner MJ. Mutational
analysis of RsrA, a zinc-binding anti-sigma factor with a thiol-disulphide redox
switch. Mol Microbiol. 2001 Feb;39(4):1036-47. PubMed PMID: 11251822.


82: Ferguson N, Li W, Capaldi AP, Kleanthous C, Radford SE. Using chimeric
immunity proteins to explore the energy landscape for alpha-helical protein
folding. J Mol Biol. 2001 Mar 16;307(1):393-405. PubMed PMID: 11243827.


83: Capaldi AP, Shastry MC, Kleanthous C, Roder H, Radford SE. Ultrarapid mixing
experiments reveal that Im7 folds via an on-pathway intermediate. Nat Struct
Biol. 2001 Jan;8(1):68-72. PubMed PMID: 11135674.


84: Carr S, Walker D, James R, Kleanthous C, Hemmings AM. Crystallization of the
cytotoxic domain of a ribosome-inactivating colicin in complex with its immunity
protein. Acta Crystallogr D Biol Crystallogr. 2000 Dec;56(Pt 12):1630-3. PubMed
PMID: 11092930.


85: Penfold CN, Garinot-Schneider C, Hemmings AM, Moore GR, Kleanthous C, James
R. A 76-residue polypeptide of colicin E9 confers receptor specificity and
inhibits the growth of vitamin B12-dependent Escherichia coli 113/3 cells. Mol
Microbiol. 2000 Nov;38(3):639-49. PubMed PMID: 11069686.


86: Boetzel R, Czisch M, Kaptein R, Hemmings AM, James R, Kleanthous C, Moore GR.
NMR investigation of the interaction of the inhibitor protein Im9 with its
partner DNase. Protein Sci. 2000 Sep;9(9):1709-18. PubMed PMID: 11045617; PubMed
Central PMCID: PMC2144694.


87: Carr S, Walker D, James R, Kleanthous C, Hemmings AM. Inhibition of a
ribosome-inactivating ribonuclease: the crystal structure of the cytotoxic domain
of colicin E3 in complex with its immunity protein. Structure. 2000 Sep
15;8(9):949-60. PubMed PMID: 10986462.


88: Kühlmann UC, Pommer AJ, Moore GR, James R, Kleanthous C. Specificity in
protein-protein interactions: the structural basis for dual recognition in
endonuclease colicin-immunity protein complexes. J Mol Biol. 2000 Sep
1;301(5):1163-78. PubMed PMID: 10966813.


89: Hannan JP, Whittaker SB, Hemmings AM, James R, Kleanthous C, Moore GR. NMR
studies of metal ion binding to the Zn-finger-like HNH motif of colicin E9. J
Inorg Biochem. 2000 Apr;79(1-4):365-70. PubMed PMID: 10830890.


90: Mavrogeni SI, Tsirintani M, Kleanthous C, Vranakis K, Secheta E, Mihelakis D,
Sotiriou D, Gatzonis M, Tsolas C, Kontaratos AN, Cokkinos DV. Supervision of
thrombolysis of acute myocardial infarction using telemedicine. J Telemed
Telecare. 2000;6(1):54-8. PubMed PMID: 10824393.


91: Bingham R, Ekunwe SI, Falk S, Snyder L, Kleanthous C. The major head protein
of bacteriophage T4 binds specifically to elongation factor Tu. J Biol Chem. 2000
Jul 28;275(30):23219-26. PubMed PMID: 10801848.


92: Whittaker SB, Czisch M, Wechselberger R, Kaptein R, Hemmings AM, James R,
Kleanthous C, Moore GR. Slow conformational dynamics of an endonuclease persist
in its complex with its natural protein inhibitor. Protein Sci. 2000
Apr;9(4):713-20. PubMed PMID: 10794413; PubMed Central PMCID: PMC2144623.


93: Whittaker SB, Boetzel R, MacDonald C, Lian LY, James R, Kleanthous C, Moore
GR. Assignment of 1H, 13C and 15N signals of the DNase domain of colicin E9. J
Biomol NMR. 1999 Jun;14(2):201-2. PubMed PMID: 10610142.


94: Kühlmann UC, Moore GR, James R, Kleanthous C, Hemmings AM. Structural
parsimony in endonuclease active sites: should the number of homing endonuclease
families be redefined? FEBS Lett. 1999 Dec 10;463(1-2):1-2. PubMed PMID:
10601625.


95: Kraal B, Lippmann C, Kleanthous C. Translational regulation by modifications
of the elongation factor Tu. Folia Microbiol (Praha). 1999;44(2):131-41. Review.
PubMed PMID: 10588048.


96: Pommer AJ, Kühlmann UC, Cooper A, Hemmings AM, Moore GR, James R, Kleanthous
C. Homing in on the role of transition metals in the HNH motif of colicin
endonucleases. J Biol Chem. 1999 Sep 17;274(38):27153-60. PubMed PMID: 10480931.


97: Hannan JP, Whittaker SB, Davy SL, Kühlmann UC, Pommer AJ, Hemmings AM, James
R, Kleanthous C, Moore GR. NMR study of Ni2+ binding to the H-N-H endonuclease
domain of colicin E9. Protein Sci. 1999 Aug;8(8):1711-3. PubMed PMID: 10452617;
PubMed Central PMCID: PMC2144424.


98: Kang JG, Paget MS, Seok YJ, Hahn MY, Bae JB, Hahn JS, Kleanthous C, Buttner
MJ, Roe JH. RsrA, an anti-sigma factor regulated by redox change. EMBO J. 1999
Aug 2;18(15):4292-8. PubMed PMID: 10428967; PubMed Central PMCID: PMC1171505.


99: Kleanthous C, James R, Hemmings AM, Moore GR. Protein antibiotics and their
inhibitors. Biochem Soc Trans. 1999 Feb;27(2):63-7. Review. PubMed PMID:
10093708.


100: Kühlmann UC, Kleanthous C, James R, Moore GR, Hemmings AM. Preliminary X-ray
crystallographic analysis of the complex between the DNAase domain of colicin E9
and its cognate immunity protein. Acta Crystallogr D Biol Crystallogr. 1999
Jan;55(Pt 1):256-9. Epub 1999 Jan 1. PubMed PMID: 10089452.


101: Kleanthous C, Kühlmann UC, Pommer AJ, Ferguson N, Radford SE, Moore GR,
James R, Hemmings AM. Structural and mechanistic basis of immunity toward
endonuclease colicins. Nat Struct Biol. 1999 Mar;6(3):243-52. PubMed PMID:
10074943.


102: Ferguson N, Capaldi AP, James R, Kleanthous C, Radford SE. Rapid folding
with and without populated intermediates in the homologous four-helix proteins
Im7 and Im9. J Mol Biol. 1999 Mar 12;286(5):1597-608. PubMed PMID: 10064717.


103: Boetzel R, Czisch M, MacDonald CJ, Kaptein R, Hemmings AM, James R,
Kleanthous C, Moore GR. Assignment of 1H, 13C and 15N signals of the inhibitor
protein Im9 bound to the DNase domain of colicin E9. J Biomol NMR. 1998
Nov;12(4):567-8. PubMed PMID: 9917143.


104: Whittaker SB, Boetzel R, MacDonald C, Lian LY, Pommer AJ, Reilly A, James R,
Kleanthous C, Moore GR. NMR detection of slow conformational dynamics in an
endonuclease toxin. J Biomol NMR. 1998 Jul;12(1):145-59. PubMed PMID: 9729794.


105: Li W, Hamill SJ, Hemmings AM, Moore GR, James R, Kleanthous C. Dual
recognition and the role of specificity-determining residues in colicin E9
DNase-immunity protein interactions. Biochemistry. 1998 Aug 25;37(34):11771-9.
PubMed PMID: 9718299.


106: Pommer AJ, Wallis R, Moore GR, James R, Kleanthous C. Enzymological
characterization of the nuclease domain from the bacterial toxin colicin E9 from
Escherichia coli. Biochem J. 1998 Sep 1;334 ( Pt 2):387-92. PubMed PMID: 9716496;
PubMed Central PMCID: PMC1219700.


107: Dennis CA, Videler H, Pauptit RA, Wallis R, James R, Moore GR, Kleanthous C.
A structural comparison of the colicin immunity proteins Im7 and Im9 gives new
insights into the molecular determinants of immunity-protein specificity. Biochem
J. 1998 Jul 1;333 ( Pt 1):183-91. PubMed PMID: 9639578; PubMed Central PMCID:
PMC1219571.


108: Kleanthous C, Hemmings AM, Moore GR, James R. Immunity proteins and their
specificity for endonuclease colicins: telling right from wrong in
protein-protein recognition. Mol Microbiol. 1998 Apr;28(2):227-33. Review. PubMed
PMID: 9622349.


109: Leech AP, Boetzel R, McDonald C, Shrive AK, Moore GR, Coggins JR, Sawyer L,
Kleanthous C. Re-evaluating the role of His-143 in the mechanism of type I
dehydroquinase from Escherichia coli using two-dimensional 1H,13C NMR. J Biol
Chem. 1998 Apr 17;273(16):9602-7. PubMed PMID: 9545291.


110: Georgiou T, Yu YN, Ekunwe S, Buttner MJ, Zuurmond A, Kraal B, Kleanthous C,
Snyder L. Specific peptide-activated proteolytic cleavage of Escherichia coli
elongation factor Tu. Proc Natl Acad Sci U S A. 1998 Mar 17;95(6):2891-5. PubMed
PMID: 9501186; PubMed Central PMCID: PMC19665.


111: Wallis R, Leung KY, Osborne MJ, James R, Moore GR, Kleanthous C. Specificity
in protein-protein recognition: conserved Im9 residues are the major determinants
of stability in the colicin E9 DNase-Im9 complex. Biochemistry. 1998 Jan
13;37(2):476-85. PubMed PMID: 9425068.


112: Garinot-Schneider C, Penfold CN, Moore GR, Kleanthous C, James R.
Identification of residues in the putative TolA box which are essential for the
toxicity of the endonuclease toxin colicin E9. Microbiology. 1997 Sep;143 ( Pt
9):2931-8. PubMed PMID: 9308177.


113: Li W, Dennis CA, Moore GR, James R, Kleanthous C. Protein-protein
interaction specificity of Im9 for the endonuclease toxin colicin E9 defined by
homologue-scanning mutagenesis. J Biol Chem. 1997 Aug 29;272(35):22253-8. PubMed
PMID: 9268373.


114: Osborne MJ, Wallis R, Leung KY, Williams G, Lian LY, James R, Kleanthous C,
Moore GR. Identification of critical residues in the colicin E9 DNase binding
region of the Im9 protein. Biochem J. 1997 May 1;323 ( Pt 3):823-31. PubMed PMID:
9169618; PubMed Central PMCID: PMC1218388.


115: Bottomley JR, Clayton CL, Chalk PA, Kleanthous C. Cloning, sequencing,
expression, purification and preliminary characterization of a type II
dehydroquinase from Helicobacter pylori. Biochem J. 1996 Oct 15;319 ( Pt
2):559-65. PubMed PMID: 8912695; PubMed Central PMCID: PMC1217804.


116: Harris JM, Gonzalez-Bello C, Kleanthous C, Hawkins AR, Coggins JR, Abell C.
Evidence from kinetic isotope studies for an enolate intermediate in the
mechanism of type II dehydroquinases. Biochem J. 1996 Oct 15;319 ( Pt 2):333-6.
PubMed PMID: 8912664; PubMed Central PMCID: PMC1217773.


117: Bottomley JR, Hawkins AR, Kleanthous C. Conformational changes and the role
of metals in the mechanism of type II dehydroquinase from Aspergillus nidulans.
Biochem J. 1996 Oct 1;319 ( Pt 1):269-78. PubMed PMID: 8870678; PubMed Central
PMCID: PMC1217764.


118: Garinot-Schneider C, Pommer AJ, Moore GR, Kleanthous C, James R.
Identification of putative active-site residues in the DNase domain of colicin E9
by random mutagenesis. J Mol Biol. 1996 Aug 2;260(5):731-42. PubMed PMID:
8709151.


119: Osborne MJ, Breeze AL, Lian LY, Reilly A, James R, Kleanthous C, Moore GR.
Three-dimensional solution structure and 13C nuclear magnetic resonance
assignments of the colicin E9 immunity protein Im9. Biochemistry. 1996 Jul
23;35(29):9505-12. PubMed PMID: 8755730.


120: James R, Kleanthous C, Moore GR. The biology of E colicins: paradigms and
paradoxes. Microbiology. 1996 Jul;142 ( Pt 7):1569-80. Review. PubMed PMID:
8757721.


121: Leech AP, James R, Coggins JR, Kleanthous C. Mutagenesis of active site
residues in type I dehydroquinase from Escherichia coli. Stalled catalysis in a
histidine to alanine mutant. J Biol Chem. 1995 Oct 27;270(43):25827-36. PubMed
PMID: 7592767.


122: Wallis R, Leung KY, Pommer AJ, Videler H, Moore GR, James R, Kleanthous C.
Protein-protein interactions in colicin E9 DNase-immunity protein complexes. 2.
Cognate and noncognate interactions that span the millimolar to femtomolar
affinity range. Biochemistry. 1995 Oct 24;34(42):13751-9. PubMed PMID: 7577967.


123: Wallis R, Moore GR, James R, Kleanthous C. Protein-protein interactions in
colicin E9 DNase-immunity protein complexes. 1. Diffusion-controlled association
and femtomolar binding for the cognate complex. Biochemistry. 1995 Oct
24;34(42):13743-50. PubMed PMID: 7577966.


124: Osborne MJ, Lian LY, Wallis R, Reilly A, James R, Kleanthous C, Moore GR.
Sequential assignments and identification of secondary structure elements of the
colicin E9 immunity protein in solution by homonuclear and heteronuclear NMR.
Biochemistry. 1994 Oct 18;33(41):12347-55. PubMed PMID: 7918457.


125: Wallis R, Reilly A, Barnes K, Abell C, Campbell DG, Moore GR, James R,
Kleanthous C. Tandem overproduction and characterisation of the nuclease domain
of colicin E9 and its cognate inhibitor protein Im9. Eur J Biochem. 1994 Mar
1;220(2):447-54. PubMed PMID: 8125102.


126: Reilly A, Morgan P, Davis K, Kelly SM, Greene J, Rowe AJ, Harding SE, Price
NC, Coggins JR, Kleanthous C. Product-induced stabilization of tertiary and
quaternary structure in Escherichia coli dehydroquinase. J Biol Chem. 1994 Feb
25;269(8):5523-6. PubMed PMID: 8119885.


127: Wallis R, Moore GR, Kleanthous C, James R. Molecular analysis of the
protein-protein interaction between the E9 immunity protein and colicin E9. Eur J
Biochem. 1992 Dec 15;210(3):923-30. PubMed PMID: 1483475.


128: Deka RK, Kleanthous C, Coggins JR. Identification of the essential histidine
residue at the active site of Escherichia coli dehydroquinase. J Biol Chem. 1992
Nov 5;267(31):22237-42. PubMed PMID: 1429576.


129: Boys CW, Bury SM, Sawyer L, Moore JD, Charles IG, Hawkins AR, Deka R,
Kleanthous C, Coggins JR. Crystallization of a type I 3-dehydroquinase from
Salmonella typhi. J Mol Biol. 1992 Sep 5;227(1):352-5. PubMed PMID: 1522599.


130: Wallis R, Reilly A, Rowe A, Moore GR, James R, Kleanthous C. In vivo and in
vitro characterization of overproduced colicin E9 immunity protein. Eur J
Biochem. 1992 Jul 15;207(2):687-95. PubMed PMID: 1633820.


131: Kleanthous C, Deka R, Davis K, Kelly SM, Cooper A, Harding SE, Price NC,
Hawkins AR, Coggins JR. A comparison of the enzymological and biophysical
properties of two distinct classes of dehydroquinase enzymes. Biochem J. 1992 Mar
15;282 ( Pt 3):687-95. Erratum in: Biochem J 1992 May 1;283(Pt 3):920. PubMed
PMID: 1554351; PubMed Central PMCID: PMC1130842.


132: Baker DP, Kleanthous C, Keen JN, Weinhold E, Fewson CA. Mechanistic and
active-site studies on D(--)-mandelate dehydrogenase from Rhodotorula graminis.
Biochem J. 1992 Jan 1;281 ( Pt 1):211-8. PubMed PMID: 1731758; PubMed Central
PMCID: PMC1130663.


133: Kleanthous C, Reilly M, Cooper A, Kelly S, Price NC, Coggins JR.
Stabilization of the shikimate pathway enzyme dehydroquinase by covalently bound
ligand. J Biol Chem. 1991 Jun 15;266(17):10893-8. PubMed PMID: 2040606.


134: Kleanthous C, Coggins JR. Reversible alkylation of an active site methionine
residue in dehydroquinase. J Biol Chem. 1990 Jul 5;265(19):10935-9. PubMed PMID:
2193028.


135: Kleanthous C, Campbell DG, Coggins JR. Active site labeling of the shikimate
pathway enzyme, dehydroquinase. Evidence for a common substrate binding site
within dehydroquinase and dehydroquinate synthase. J Biol Chem. 1990 Jul
5;265(19):10929-34. PubMed PMID: 2193027.


136: Nimmo HG, Douglas F, Kleanthous C, Campbell DG, MacKintosh C. Identification
of a cysteine residue at the active site of Escherichia coli isocitrate lyase.
Biochem J. 1989 Jul 15;261(2):431-5. PubMed PMID: 2673221; PubMed Central PMCID:
PMC1138844.


137: Lewendon A, Murray IA, Kleanthous C, Cullis PM, Shaw WV. Substitutions in
the active site of chloramphenicol acetyltransferase: role of a conserved
aspartate. Biochemistry. 1988 Sep 20;27(19):7385-90. PubMed PMID: 3061455.


138: Kleanthous C, Wemmer DE, Schachman HK. The role of an active site histidine
in the catalytic mechanism of aspartate transcarbamoylase. J Biol Chem. 1988 Sep
15;263(26):13062-7. PubMed PMID: 3047117.


139: Kleanthous C, Cullis PM, Shaw WV. 3-(Bromoacetyl)chloramphenicol, an active
site directed inhibitor for chloramphenicol acetyltransferase. Biochemistry. 1985
Sep 24;24(20):5307-13. PubMed PMID: 3865688.


140: Kleanthous C, Shaw WV. Analysis of the mechanism of chloramphenicol
acetyltransferase by steady-state kinetics. Evidence for a ternary-complex
mechanism. Biochem J. 1984 Oct 1;223(1):211-20. PubMed PMID: 6594136; PubMed
Central PMCID: PMC1144282.
 

 





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