Flow Cytometry Facility Section
There is a Becton Dickinson FACSCalibur with a single 488nm blue laser and the following optical filters:
FSC: 488/10nm band pass
SSC: 488/10nm band pass
FL1: 530/30nm band pass
FL2: 585/42nm band pass
FL3: 670 long pass
CellQuest Pro software (version 5.2.1) is available on the attached computer for acquisition and analysis.
As the machine is housed in a tissue culture facility any yeast or bacteria must be killed before being brought into the room.
Please use, shut down and leave the machine using the protocols displayed on the front of the machine, to help maintain this equipment in good working order. You need to take copies of your data from the machine as it is not backed up.
Equipment location: Rodney Porter Building, 20.25 Containment Level 2
Flow cytometer/cell sorter for microbial cells
It is a Bio-Rad S3e, which is fairly easy to operate.
It is suitable for bacteria and yeast cell sorting, isolating living cells according to specific fluorescent staining.
It is not for mammalian cells, pathogens or clinical samples.
Please contact firstname.lastname@example.org to organise training and use.