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An
Advanced Practical Course in NMR Techniques for Membrane Proteins
Rutherford
Appleton Laboratory
11th
- 14th May, 2009
Lectures:
Anja
Böckmann 1. Deuteration for solution and solid
state NMR.
2. Aspects of protein assignment strategies
and structure calculation.
Clemens
Glaubitz To
be announced.
Victoria A. Higman 1. Protein assignment, experiment types,
labelling strategies, data management of large membrane systems using
solid-state MAS NMR.
2. CCPNmr Analysis software and its use
for assignment.
David
Middleton 1. Methods of rendering proteins into a
form for high resolution solid state NMR.
2. Small molecule studies of membrane
receptors.
Alain Milon 1. Labeling strategies and structural
resolution of beta-barrel porins by solution state NMR: A. Milon
2. Structural resolution of beta barrel
porins by solution state NMR: practical based on real experimental NMR spectra,
assignment, restrained molecular dynamics: M. Renault,
Niels Nielsen 1. Using SIMPSON.
2. Solid state NMR theory and pulse
sequence for membrane protein analysis.
Hartmut Oschkinat 1.
Successes of solid state NMR in
resolving structures of membrane proteins and dynamical complexes
Practical demonstrations:
Anja
Böckmann Protocols
how to achieve high degrees of deuteration for proteins.
Clemens
Glaubitz To
be announced.
Victoria
A. Higman Solution
and solid-state MAS NMR data collection/instrument set up and structure
calculations / restraint generation.
Alain
Milon MACADAM
for membrane peptides.
David
Middleton Tips
on how to make nanocrystals, amorphous and lyophilized samples for high
resolution solid state NMR studies.
Niels
Nielsen Use
of SIMPSON.
Hartmut
Oschkinat To
be announced
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Lectures & Demonstrations |
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