As well as the usual standard biochemical technquies being available, the Intsitute is well equipped with a range of facilities more specific to glycobiology research.

Joint Institute / Department of Biochemistry Facilities --

Analytical ultracentrifuge - Further information - (contact Russell Wallis)
The departmental analytical ultracentrifuge (Beckman XL-A 70) is housed in the Glycobiology Institute and is available for use by members of the department of Biochemistry. The centrifuge is equipped with absorbance optics and software is installed for the analysis of both sedimentation and equilibrium experiments.
 
This facility is not run as a service but training can be arranged if required (one person per research group) both in setting up centrifuge runs and in the use of the data analysis software.
 
Flow Cytometry - (contact Gabriele Reinkensmeier)
Two Becton Dickinson flow cytometers, a FACScan and a FACSCalibur, are available for use by members of Biochemistry and other University Departments. Up to three colour analyses are possible with a cell sorting option.
 
This facility is not offered as a service but full training can be arranged. For further information and to book this facility contact Gabi Reinkensmeier, 75724 or gabi@oxglua.glycob.ox.ac.uk.

Amino acid analysis - (contact Brian Matthews)
PKO TAG workstation

BioGel P4 chromatography, separation of oligosaccharides by hydrodynamic volume- (contact Brian Matthews)
Large scale - manual systems
Small scale - 2 x GlycoMap automated systems

Cell culture facilities - (contact Fran Platt)

Computing and computer modelling - (contact Mark Wormald)

SUN Ultra 1 - institute server
Silicon Graphics Indigo II EX 10000
Insight 2000 and Discover (molecular modelling)
Molscript (molecular display) - documentation
Felix (NMR data processing)
Morass and Conmor (NOE matrix calculations)
Networked Printers
HP LaserJet 5Si MX - B&W laser printer, A3 and A4, 600 dpi
QMS ColourScript 230 - Colour thermal wax transfer, A3 and A4, 300 dpi
Epson Stylus Colour 1160 - Colour inkjet, A3 and A4, 300 dpi
Network Slide-Maker
Polaroid ProPallette 7000 - accessible from all PCs and Macs
Slide scanner and Arcus II flat-bed scanner
Connected to a PowerMac with MicroSoft Office
and Adobe Photoshop software
 
Dionex chromatography - (contact Terry Butters)

Gas Chromatography - (contact Brian Matthews)

Packed capillary column instruments with JCD, FID, MS and radioactivity detectors
 
Glycosidase enzyme bank - (contact Terry Butters)

High Pressure Liquid Chromatography - (contact Louise Royle)

Four HPLC systems equipped with flouorescence detectors
Columns - GlycoSep-C (WAX), GlycoSep-N
Waters Millenium software
 
Hydrazinolysis, chemical release of protein linked glycans - (contact Brian Matthews)
Large scale - manual system
Small scale - 2 x GlycoPrep 1000 automated systems
 
Liquid scintillation counting - (contact Brian Matthews)
Beckman 3801
 
Mass Spectrometry - (contact David Harvey)
Thermo Bioanalysis LaserMat 2000
Matrix-assisted laser desorption (MALDI) time-of-flight (TOF) mass spectrometer.
Micromass AutoSpec QFPD
Magnetic sector mass spectrometer fitted with an array detector and a tandem quadrupole analyser. Ionisation techniques available include electron-impact, fast-atom bombardment (FAB, LSIMS) and MALDI (nitrogen laser, 337 nM). Capilary GC/MS is also available on this instrument.
Micromass TofSpec
2E MALDI reflectron-TOF mass spectrometer
Micromass Q-Tof
Electrospray mass spectrometer interfaced to a Waters Cap-LC liquid chromatography system.
 
Nuclear Magnetic Resonance Spectroscopy - (contact Mark Wormald)
Varian UnityInova 500
3 rf channels + 2 waveform generators + performa II gradients
Proton/Flourine, Broad Band, Indirect Detection probes and H/C/N PFG probe
Sun Ultra 10 host computer
Sun SPARC 5/110 reprocessing computer