Instrument

Technique

Abbreviation

Wyatt HELEOS

Size exclusion chromatography Multiple Angle Light Scatter

SEC MALS

Beckman XL-I

Analytical ultracentrifugation

AUC

Viscotek 802

Dynamic light scatter

DLS

Malvern Vcap

Differential scanning calorimetry

DSC

JASCO 815

Circular Dichroism

CD

StratageneMC3005PCR

Differential scanning fluorimetry

Thermofluor

Monolith 11.5

Microscale Thermophoresis

MST

Biacore T200

Surface plasmon resonance

SPR

Microcal iTC200

Isothermal titration calorimetry

ITC

PE LS50-B

Fluorimetry

Fluorimetry

Applied PhotophysicsSX

Stopped flow

Stopped flow

About The Molecular Biophysics Suite

The Molecular Biophysics Suite provides a wide range of biophysical techniques for researchers. It is available to researchers in the University of Oxford, and external users both academic and commercial.

We are experts at characterising recombinant proteins and their interactions in solution.

The facility is equipped with instrumentation to characterise the solution state, stability, interactions and kinetics, and can offer help with planning experiments and their analysis, as well as training on particular techniques.

Location

New Biochemistry building, rooms 00-059 and 00-064.

Contact and further information

Please contact the facility manager David Staunton to discuss potential projects and charges.

david.staunton@bioch.ox.ac.uk

Tel. 01865 613328

External Work

We have worked with many local bioscience and pharmaceutical companies over the past eight years and are happy to discuss possible collaborations.

These publications illustrate some of the applications of our techniques and the figures above are from or derived from them.

Publications

Hatzopoulos, GN. et al. Structural Analysis of the G-Box Domain of the Microcephaly Protein CPAP Suggests a Role in Centriole Architecture. (2013). Structure 21 2069-2077.

Wright,KE. et al. Structure of malaria invasion protein RH5 with erythrocyte basigin and blocking antibodies.(2014). Nature 515 427. doi: 10.1038

van der Veen, S. et al. Nonfunctional Variant 3 Factor H Binding Proteins as Meningococcal Vaccine Candidates. (2013) Infect. Immun. 2014, 82(3) 1157-1163

Lane-Serff H. et al. Structural basis for ligand and innate immunity factor uptake by the trypanosome haptoglobin-haemoglobin receptor. (2014). Elife.10.7554/eLife.05553

Rogala, KB. et al. The Caenorhabditis elegans protein SAS-5 forms large oligomeric assemblies critical for centriole formation. (2015) eLife;10.7554/eLife.07410

Grabarczyk DB, Berks BC. Intermediates in the Sox sulfur oxidation pathway are bound to a sulfane conjugate of the carrier protein SoxYZ. (2017) PLoS One. Mar 3;12(3):e0173395. doi: 10.1371