Department of Biochemistry University of Oxford Department of Biochemistry
University of Oxford
South Parks Road
Oxford OX1 3QU

Tel: +44 (0)1865 613200
Fax: +44 (0)1865 613201
Image showing the global movement of lipids in a model planar membrane
Matthieu Chavent, Sansom lab
Anaphase bridges in fission yeast cells
Whitby lab
Lactose permease represented using bending cylinders in Bendix software
Caroline Dahl, Sansom lab
Epithelial cells in C. elegans showing a seam cell that failed to undergo cytokinesis
Serena Ding, Woollard lab
Collage of Drosophila third instar larva optic lobe
Lu Yang, Davis lab
First year Biochemistry students at a practical class
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Research Facilities

The Department has some of the best-equipped facilities in the country, supporting researchers in a number of areas including biophysics, high-resolution microscopy, NMR, protein crystallisation and proteomics.

Lab Panorama

Our equipment is available to researchers in Biochemistry and other departments within the University, and also to those working in industry. The Molecular Biophysics Suite, for example, provides an important service to local biotech and pharma companies who benefit from both the infrastructure and technical expertise offered.

The service and expertise we provide is tailored to suit the needs of users. Formal training courses or informal one-to-one training is offered for a number of the facilities including the Molecular Biophysics Suite, Micron and NMR.

For general enquiries about the facilities and to discuss your needs in more details, please contact us (david.staunton@bioch.ox.ac.uk or 01865 613328). Details about the equipment and its use can be found below, under the individual facility.

The Department also has strong links to many other facilities within the University and more widely in the area. These include:

Comprehensive information about research equipment and facilities available across the University, some of which is restricted to internal users, can be found here.

Molecular Biophysics Suite

We offer a range of techniques to study macromolecules and macromolecular complexes. These include analytical ultracentrifugation, differential scanning calorimetry, electrospray ionization mass spectrometry, isothermal titration calorimetry and surface plasmon resonance. All users must be registered with Dr David Staunton and receive any necessary training.

Contact – Dr David Staunton (david.staunton@bioch.ox.ac.uk; 01865 (6)13328)

Further information -
http://www.bioch.ox.ac.uk/aspsite/index.asp?sectionid=biophysicsfacility

Micron Oxford Advanced Bioimaging Unit

Micron Oxford is a multidisciplinary unit focusing on the development and use of single molecule methods, light sheet and super-resolution microscopy. It is located within the Department of Biochemistry and the Dunn School of Pathology.

Contact – micron@bioch.ox.ac.uk

Further information - http://www.micron.ox.ac.uk/microngroup/home.php

NMR

The NMR facility houses four solution-state spectrometers operating at 1H frequencies of 500, 600, 750 and 950 MHz. The 500 and 600 MHz spectrometers have Bruker consoles with triple-resonance TCI Cryoprobes. The facility is located in the basement of the Rex Richards Building close to the New Biochemistry building. All users must be registered and trained to use the facility.

Contact – Professor Christina Redfield (christina.redfield@bioch.ox.ac.uk; 01865 (2)75330)

Further information - http://www.bioch.ox.ac.uk/aspsite/index.asp?sectionid=nmrfacility

Protein crystallisation

The Crystallisation Facility houses robotic systems for high throughput macromolecular crystallisation and gives users access to a broad range of crystallisation screens, additives and visualisation instruments. The robotic instruments include:

  • TTP Mosquito for the preparation of crystallisation trials.
  • ArtRobbins LCP Gryphon for the preparation of lipidic cubic phase crystal trials.
  • Rigaku Minstrel HTUV crystallisation imager with integrated robotic imagers, allowing the storage and automatic imaging of crystals at 4C or 18C using both visible and UV light for visualisation.

The facility also contains a variety of microscopes for the visualisation and harvesting of crystals, cold room facilities, and capacity for the storage of cryo-cooled crystalline samples.

The facility manager, Dr Ed Lowe, can offer assistance with both the preparation of crystallisation trials and with the processing of data following diffraction experiments.

Contact – Dr Ed Lowe (edward.lowe@bioch.ox.ac.uk; 01865 (6)13288)

(internal users can find out more on the crystallisation facility wiki

Advanced Proteomics Facility

We provide access to cutting edge mass spectrometry and have experience in protein identification, quantitative mass spectrometry, and characterisation methods for a range of post-translational modifications. Our facility is equipped with a wide range of instrumentation, sample preparation technologies and data analysis software.

Lab Panorama

Please always contact us prior to commencing a new project in order to discuss project aims and requirements.

Contact – Professor Shabaz Mohammed (shabaz.mohammed@chem.ox.ac.uk. Click here for personal page.

The main facility website is available here.

Flow Cytometry Facilities:

A Becton and Dickenson 3-colour flow cytometer (FACScalibur) is available, which has cell sorting ability. Based in Rodney Porter Building, 20.25 Containment Level 2. More Details

Also available:
 
A Bio-Rad S3e Flow cytometer/cell sorter for microbial cells, which is fairly easy to operate, see http://www.bio-rad.com/en-uk/product/s3e-cell-sorter
 
It is suitable for bacteria and yeast cell sorting, isolating living cells according to specific fluorescent staining. 
 
It is not for mammalian cells, pathogens or clinical samples.
 
Please contact mark.howarth@bioch.ox.ac.uk to organise training and use.

 

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