We are molecular detectives who use interdisciplinary approaches to understand how the bacterial chromosome is organized, replicated, repaired, unlinked and segregated within a living cell. Furthermore, we study how these processes are interwoven with cell physiology in steady state growth and under ‘perturbed’ conditions. For example, in response to treatment with a range of antibiotics. The research uses quantitative and super-resolution live cell imaging to observe the positions and behaviour of chromosome processing molecular machines as cells proceed through growth and division cycles. Normal behaviour is perturbed by genetics, inhibitors and other means, thereby enabling ‘in vivo’ biochemistry. Imaging is complemented by single-molecule and ensemble in vitro biochemistry, genetics, and by methods that capture chromosome conformation in vivo and provide insight into the patterns of protein binding to chromosomes.