Department of Biochemistry University of Oxford Department of Biochemistry
University of Oxford
South Parks Road
Oxford OX1 3QU

Tel: +44 (0)1865 613200
Fax: +44 (0)1865 613201
Image showing the global movement of lipids in a model planar membrane
Matthieu Chavent, Sansom lab
Anaphase bridges in fission yeast cells
Whitby lab
Lactose permease represented using bending cylinders in Bendix software
Caroline Dahl, Sansom lab
Epithelial cells in C. elegans showing a seam cell that failed to undergo cytokinesis
Serena Ding, Woollard lab
Collage of Drosophila third instar larva optic lobe
Lu Yang, Davis lab
First year Biochemistry students at a practical class
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Jason Schnell
Structure and function of membrane proteins and their interactions with each other and lipid bilayers

Co-workers: Felipe Ossa, Muhd Mohd Kipli, Frederik Thomasen, Alex Jiang.


We are interested in understanding the structure and function of membrane proteins, especially those implicated in human health and disease. More specifically, our research aims to identify the molecular basis of the interactions between membrane proteins and the components of the membrane bilayer (e.g., lipids and cholesterol), other proteins, and drug-like small molecules; ultimately, we are interested in how these interactions lead to events on the cellular level. Current research is focused on the following systems:

Protein-protein and protein-membrane interactions of the Influenza virus: The influenza virus is easily spreadable between people and can result in severe illness and death, particularly for young children and older adults. Globally, 3-5 million people suffer severe illness and 250,000-500,000 people die from influenza every year (World Health Organization estimates). We are studying the protein-protein and lipid interactions of ‘flu’ proteins to better understand their role in the virus life cycle and to identify potential therapeutic targets. Our aim is to facilitate this research by providing a greater understanding of the S1R structure and dynamics, and its interactions with other proteins and small molecules.



DP1/Reticulon family: The DP1/Reticulon family of integral membrane proteins are responsible for maintaining the high membrane curvature of the tubular ER, and mutations in these proteins are implicated in neuronal diseases such as Hereditary Spastic Paraplegia. To gain insight into how this class of proteins curve and remodel lipid membranes – and how they malfunction in disease – we have focused on the protein Yop1p, which is a DP1 family member from Saccharomyces cerevisiae.

A central technique of our laboratory is solution nuclear magnetic resonance (NMR) spectroscopy, which allows atomic-level studies of protein structures and their interactions with lipids and small molecules. NMR can be uniquely informative in situations where the molecular conformations or interactions are dynamic or heterogeneous. However, a wide variety of biochemical and biophysical tools are brought to bear on the research questions, including electron microscopy, circular dichroism, fluorescence, electron microscopy, and analytical ultracentrifugation. We also have collaborations with various research groups around the world including virologists, cell biologists, and computational biologists.    


Recent Publications

  1. Chipot, C., Dehez, F., Schnell, J. R., Zitzmann, N., Pebay-Peyroula, E., Catoire, L. J., Miroux, B., Kunji, E. R. S., Veglia, G., Cross, T. A., Schanda, P. (2018) Perturbations of native membrane protein structure in alkyl phosphocholine detergents: A critical assessment of NMR and biophysical studies. Chem. Rev., Feb 28. doi: 10.1021/acs.chemrev.7b00570. (Epub ahead of print) [PMID: 29488756]
  2. Ossa, F., Schnell, J. R., Ortega-Roldan J. L. (2017) A review of the human sigma-1 receptor structure. Adv. Exp. Med. Biol., 964:15-29. [PMID: 28315262]
  3. Breeze, E., Dzimitrowicz, N., Kriechbaumer, V., Brooks, R., Botchway, S. W., Brady, J. P., Hawes. C., Dixon, A., Schnell, J. R., Fricker, M. D. and Frigerio, L. (2016) A C-terminal amphipathic helix is necessary for the in vivo tubule-shaping function of a plant reticulon. Proc Natl Acad Sci U S A, 113(39):10902-7. [PMID: 27621477]
  4. Ma, J., Domicevica, L., Schnell, J. R., Biggin, P. C. (2015) Position and orientational preferences of drug-like compounds in lipid membrane: A computational and NMR approach. Phys. Chem. Chem. Phys., 17(30):19766-76. [PMID: 26153345]
  5. Ortega-Roldan, J. L., Ossa, F., Amin, N. T., Schnell, J. R. (2015) Solution NMR studies reveal the location of the second transmembrane domain of the human sigma-1 receptor. FEBS Lett., 589(5):659-65. [PMID: 25647032]
  6. Brady, J. P., Claridge, J. K., Smith, P. G., Schnell, J. R. (2015) A conserved amphipathic helix is required for membrane tubule formation by Yop1p. Proc Natl Acad Sci U S A., 112(7):E639-48. [PMID: 25646439]
  7. Dixon, E. V., Claridge, J. K., Harvey, D. J., Baruah, K., Yu, X., Vesiljevic, S., Mattick, S., Pritchard, L. K., Krishna, B., Christopher N. Scanlan, C. N., Schnell, J. R., Higgins, M. K., Zitzmann, N., Crispin, M. (2014) Fragments of Bacterial Endoglycosidase S and Immunoglobulin G Reveal Subdomains of each that Contribute to Deglycosylation. J. Biol. Chem. 16;289(20):13876-89. [PMID: 24668806]
  8. Claridge, J. K, Aittoniemi, J., Cooper, D. M., and Schnell J. R. (2013) Isotropic Bicelles Stabilize the Juxtamembrane Region of the Influenza M2 Protein for Solution NMR Studies. Biochemistry. 52(47), 8420-9. [PMID: 24168642]
  9. Ortega-Roldan, J. L., Ossa, F., and Schnell, J. R. (2013) Characterization of the human Sigma-1 receptor chaperone domain structure and BiP interactions. J. Biol. Chem. 288(29), 21448-57. [PMID: 23760505]
  10. Rodriguez, F., Rouse, S. L., Tait, C., Harmer, J., De Riso, A., Timmel, C. R., Sansom, M. S. P., Berks, B. C., and Schnell, J. R. (2013) Structural model for the protein-translocating element of the twin-arginine transport system. Proc. Natl. Acad. Sci. USA, 110(12), E1092. [PMID: 23471988]
  11. Claridge, J.K. and Schnell, J. R. (2012). Bacterial production and solution NMR studies of a viral membrane ion channel. Methods Mol. Biol. 831, 165-79. [PMID: 22167674]
Full List of Publications


Lab website:

Graduate Student and Postdoctoral Positions: Enquiries with CV are welcome, and applications for FEBS, EMBO, or other fellowships are encouraged.


Research Images




Figure 1: Schematic of a construct of the influenza M2 protein solubilised in bicelles containing lipid and detergent, which enables observation of protein-lipid interactions.






Figure 2: Schematics showing the secondary structure determined for the Sigma-1 Receptor (top) and Yop1p (bottom) based on a combination of experimental and predicted information.